Table 1

Comparison of critical elements in bitrophic feeding trials of both studies

Critical elements

Alvarez-Alfageme et al. [8]

Schmidt et al. [1]


Parameter/methods

Number of test insects

Not clear, probably 34 to 41

30

Number of replicates

Not clear, probably 1

4

Total number of test insects per treatment

N = 34 to 41

N = 120

Diet treatment

Water/sugar solution (2 droplets) with Bt toxin

Meal moth eggs treated with Bt toxin in buffer solution

Diet control

Water/sugar solution (no buffer added)

Untreated meal moth eggs

Meal moth eggs treated with empty constructs in buffer solution

Meal moth eggs treated with buffer solution (3 separate control treatments; see below)

Dose(s) of administered Bt toxins

1 each

3 each (for each Bt-concentration treatment, a separate control was carried out; 3 control treatments)

Exposure time (per instar) to Bt toxin

4 × 1 day (1 day during each instar followed by recovery periods)

Total development time 12 days (Bt) to 17 days (other treatments)

9 to 10 days continuous (all instars)

Total development time 9 to 10 days

Exposed life stages

Partially, all 4 instars

Continuously, all 4 instars

ELISA with A. bipunctata larvae to verify uptake

No

No

Quantification of ingested amount of Bt toxin

No

No

Results

Control mortality (%)

Number of control treatments = 1

Number of control treatments = 3 + 3 pBD10 = 6

L1-P = 15.0%

L1-P = 15.8%, 21.7%, 10.8% (15.0%, 13.3%, 10.8%)

L1-A = 16.7%, 21.7%, 14.2% (15.8%, 15.0%, 14.2%)

Treatment mortality (%)

1 dose of each Bt toxin

3 doses of each Bt toxin

Cry1Ab

45

5

25

50

concentration

μg/ml

μg/ml

μg/ml

μg/ml

L1-P

20.6%

26.7%

48.3%

35.8%

P

3.7%

1.2%

1.6%

0

L1-A

24.3%

27.5%

49.2%

35.8%

Cry3Bb

200

5

25

50

concentration

μg/ml

μg/ml

μg/ml

μg/ml

L1-P

17.1

21.7%

32.5%

30.0%

P

0

0

1.2%

1.2%

L1-A

17.1

21.7%

33.5%

30.8%


ELISA, enzyme-linked immunosorbent assay.

Hilbeck et al. Environmental Sciences Europe 2012 24:10   doi:10.1186/2190-4715-24-10

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