Table 3

Effects of NM-105 in the earthworm reproduction test

Test and test start

Test concentration (mg/kg sdm)

Mortality (%)

Biomass per vessel at test start (g) ± SD

Biomass per vessel at test end (g) ± SD

Increase in biomass (%)

Number of juveniles per test vessel ± SD

SD (%)

Effect on reproduction (%)


Test 1 - January 2010

0 (control)

0

3.29 ± 0.24

5.47 ± 0.36

67

212 ± 46

21.7

-

50

0

3.37 ± 0.43

5.80 ± 0.15

74

295 ± 44

14.9

-39.2a**

100

0

3.49 ± 0.13

5.47 ± 0.10

57

299 ± 74

14.7

-41.0a**

200

0

3.45 ± 0.14

5.47 ± 0.11

59

315 ± 42

13.3

-48.6a**

Test 2 - May 2010

0 (control)

0

3.81 ± 0.30

5.37 ± 0.34

41

340 ± 39

11.5

-

50

0

3.62 ± 0.10

5.09 ± 0.20

41

341 ± 33

9.7

-0.3a

100

0

3.66 ± 0.11

5.66 ± 0.33

55

343 ± 28

8.2

-0.9a

200

0

3.58 ± 0.11

5.17 ± 0.40

44

290 ± 24

8.3

14.7

500

0

3.54 ± 0.08

5.24 ± 0.40

48

253 ± 62

24.5

25.5

1,000

0

3.63 ± 0.23

5.57 ± 0.26

54

319 ± 43

13.5

6.5

Test 3 - January 2011

0 (control)

0

3.70 ± 0.26

5.26 ± 0.40

42

208 ± 15

7.2

-

50

0

3.68 ± 0.16

5.36 ± 0.08

46

239 ± 22

9.2

-8.8a*

100

0

3.57 ± 0.20

5.39 ± 0.19

52

252 ± 15

6.0

-14.9a**

200

0

3.46 ± 0.09

5.58 ± 0.24

61**

265 ± 31

11.7

-27.4a**

500

0

3.59 ± 0.22

5.37 ± 0.46

50

238 ± 11

4.6

-14.4a**

750

0

3.58 ± 0.12

5.43 ± 0.36

52

279 ± 27

9.8

-34.1a**

1,000

0

3.43 ± 0.18

5.29 ± 0.53

54

286 ± 21

7.3

-37.5a**


aNegative values indicate stimulation. sdm, soil dry matter; SD, standard deviation. Asterisks indicate a statistically significant difference to controls (*0.05 ≥ p ≥ 0.01; **0.01 ≥ p ≥ 0.001).

Schlich et al. Environmental Sciences Europe 2012 24:5   doi:10.1186/2190-4715-24-5

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